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Role of hydrolase from Trichoderma Harzianum and Trichoderma Koningii in pathogenic fungus F. oxysporum inhibition

Abstract:  The genus Trichoderma is a very large group of microorganisms that play a significant role in plant protection.  Several  Trichoderma spp.  like Trichoderma harzianum and Trichoderma konigii strongly affect plants by stimulating plant growth  and by protecting plants from fungal and bacterial pathogens such as Fusarium oxysporium, being  used as a biological plant protection with the role of bio fungicides. Members of Trichoderma spp. are also used in different branches of industry, primarily for the production of of enzymes, antibiotics and other metabolites. In this study we focused on the effect of T. harzianum strain ICCF 417 and T. konigii strain ICCF 418 on F. Oxysporum (ZUM 2407) using microbiological and enzymatic tests. The results of fungal growth speed on malt medium showed that the fungus T. koningii was the fastest in growth, followed by T. harzianum and F. oxysporium after 72 hours of culture.  The results of the study showed the susceptibility of bio-fungi on the production of the enzyme FPase was 32.3% in T. harzianum comparative to T. koningii after 14 days of fermentation, the amylase was 84.5% in T. harzianum comparative to T. koningii while the CMCase was 36.6% in T. harzianum comparative to T. koningii.

Our results showed that hydrolase activities studied in this experiment play an important role in the pathogenic fungus F. oxysporum inhibition and the degree of effect is different .

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